Difference between revisions of "Part:BBa K209473"
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<partinfo>BBa_K209473 short</partinfo> | <partinfo>BBa_K209473 short</partinfo> | ||
− | expression vector backbone | + | This is an expression vector backbone, in which AarI A-D sites have been inserted into the MCS. |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | pcDNA3.1(+) is a vector designed for high-level, constitutive expression in a variety of mammalian cell lines. | ||
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Revision as of 21:18, 21 October 2009
pcDNA3.1(+) with AarI A-D sites in MCS
This is an expression vector backbone, in which AarI A-D sites have been inserted into the MCS.
Usage and Biology
pcDNA3.1(+) is a vector designed for high-level, constitutive expression in a variety of mammalian cell lines.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 952
Illegal XbaI site found at 1065
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 952
Illegal NheI site found at 895
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387
Illegal NotI site found at 978 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 952
Illegal BglII site found at 12
Illegal BamHI site found at 929
Illegal XhoI site found at 1059 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 952
Illegal XbaI site found at 1065
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 952
Illegal XbaI site found at 1065
Illegal SpeI site found at 249
Illegal SpeI site found at 935
Illegal PstI site found at 957
Illegal PstI site found at 2387
Illegal NgoMIV site found at 1497
Illegal NgoMIV site found at 2838
Illegal NgoMIV site found at 3121
Illegal AgeI site found at 1013 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 882
Illegal BsaI.rc site found at 4646
Illegal SapI site found at 3566
Illegal SapI.rc site found at 2687
Illegal SapI.rc site found at 2897