Difference between revisions of "Part:BBa K5526008"
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<partinfo>BBa_K5526008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5526008 SequenceAndFeatures</partinfo> | ||
+ | <html lang="en"> | ||
+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>Anti-PD-L1 (BBa_K5526008) Documentation</title> | ||
+ | <style> | ||
+ | body { | ||
+ | font-family: Arial, sans-serif; | ||
+ | line-height: 1.6; | ||
+ | } | ||
+ | figure { | ||
+ | text-align: center; | ||
+ | margin-bottom: 20px; | ||
+ | } | ||
+ | figcaption { | ||
+ | margin-top: 5px; | ||
+ | font-style: italic; | ||
+ | } | ||
+ | img { | ||
+ | width: 60%; /* Default width, adjustable */ | ||
+ | height: auto; /* Maintain aspect ratio */ | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
− | < | + | <h2>Part: BBa_K5526008 (Anti-PD-L1)</h2> |
− | === | + | |
− | < | + | <h3>Profile</h3> |
− | <!-- --> | + | <p><strong>Name:</strong> Anti-PD-L1</p> |
+ | <p><strong>Base Pairs:</strong> 372bp</p> | ||
+ | <p><strong>Origin:</strong> Cell, synthesized</p> | ||
+ | <p><strong>Properties:</strong> Anti-PD-L1 is an immune checkpoint inhibitor that blocks the recognition of PD-L1 expressed by tumor cells with T cells, enhancing the immune system's effect on tumors. It has been widely used in the clinical treatment of various types of cancer.</p> | ||
+ | |||
+ | <h3>Usage and Biology</h3> | ||
+ | <p>PD-L1 is a protein expressed on the surface of many tumor cells that allows them to evade the immune system by binding to the PD-1 receptor on T cells. By blocking the interaction between PD-L1 and PD-1, Anti-PD-L1 reactivates the T cell-mediated immune response, helping the immune system recognize and destroy tumor cells. It has shown remarkable therapeutic effects, particularly in the field of immunotherapy.</p> | ||
+ | |||
+ | <!-- Figure 1 --> | ||
+ | <figure> | ||
+ | <img src="https://static.igem.wiki/teams/5526/bba-k5526008/1.png" alt="Gene map of Anti-PD-L1"> | ||
+ | <figcaption>Figure 1. Gene map of Anti-PD-L1</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <h3>Cultivation, Purification, and SDS-PAGE</h3> | ||
+ | <p>AntiPD-L1 is a set gene that encodes an immunomodulatory protein, which activates the immune system to kill tumor cells. pUC57-mini is the plasmid skeleton. We applied PCR to amplify the antiPD-L1 gene (372bp) and pUC57-plldr (3150bp), and used agarose gel electrophoresis to confirm the PCR products. The result is that antiPD-L1 has a length of 372bp, as shown in Figure 2.</p> | ||
+ | |||
+ | <!-- Figure 2 --> | ||
+ | <figure> | ||
+ | <img src="https://static.igem.wiki/teams/5526/bba-k5526008/2.jpg" alt="PCR production identification by agarose gel electrophoresis"> | ||
+ | <figcaption>Figure 2. The identification of PCR production by agarose gel electrophoresis. Left: The graph shows that plactate 1 has a length of 3150 bp. Right: The graph shows that antiPD-L1 has a length of 372bp.</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <p>After optimizing the protein expression conditions, the constructed strains were expanded for culture. 5mM lactic acid was used to induce tumor drug expression in EcN1917. The experimental results are shown in Figure 3. The size of the Anti-PD-L1 protein is about 24kDa, and a large amount of target protein was successfully expressed.</p> | ||
+ | |||
+ | <!-- Figure 3 --> | ||
+ | <figure> | ||
+ | <img src="https://static.igem.wiki/teams/5526/bba-k5526008/3.jpg" alt="Detection of Anti-PD-L1 protein expression by SDS-PAGE"> | ||
+ | <figcaption>Figure 3. Detection of Anti-PD-L1 protein expression by SDS-PAGE</figcaption> | ||
+ | </figure> | ||
+ | |||
+ | <p>Future studies will focus on investigating the expression and tumor inhibition effects of the constructed probiotics in a tumor environment.</p> | ||
+ | |||
+ | </body> | ||
+ | </html> |
Latest revision as of 06:10, 29 September 2024
Anti-PD-L1
Anti-PD-L1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 25
Illegal AgeI site found at 364 - 1000COMPATIBLE WITH RFC[1000]
Part: BBa_K5526008 (Anti-PD-L1)
Profile
Name: Anti-PD-L1
Base Pairs: 372bp
Origin: Cell, synthesized
Properties: Anti-PD-L1 is an immune checkpoint inhibitor that blocks the recognition of PD-L1 expressed by tumor cells with T cells, enhancing the immune system's effect on tumors. It has been widely used in the clinical treatment of various types of cancer.
Usage and Biology
PD-L1 is a protein expressed on the surface of many tumor cells that allows them to evade the immune system by binding to the PD-1 receptor on T cells. By blocking the interaction between PD-L1 and PD-1, Anti-PD-L1 reactivates the T cell-mediated immune response, helping the immune system recognize and destroy tumor cells. It has shown remarkable therapeutic effects, particularly in the field of immunotherapy.
Cultivation, Purification, and SDS-PAGE
AntiPD-L1 is a set gene that encodes an immunomodulatory protein, which activates the immune system to kill tumor cells. pUC57-mini is the plasmid skeleton. We applied PCR to amplify the antiPD-L1 gene (372bp) and pUC57-plldr (3150bp), and used agarose gel electrophoresis to confirm the PCR products. The result is that antiPD-L1 has a length of 372bp, as shown in Figure 2.
After optimizing the protein expression conditions, the constructed strains were expanded for culture. 5mM lactic acid was used to induce tumor drug expression in EcN1917. The experimental results are shown in Figure 3. The size of the Anti-PD-L1 protein is about 24kDa, and a large amount of target protein was successfully expressed.
Future studies will focus on investigating the expression and tumor inhibition effects of the constructed probiotics in a tumor environment.