Difference between revisions of "Part:BBa K5291028:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. | + | We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. NadM encoded by nadM catalyze the adenylylation of the nicotinic acid mononucleotide precursor to nicotinic acid dinucleotide followed by its amidation to NAD+. Given the properties of this enzyme, we decided to use NadM to increase the total amount of NAD+ within the engineered bacteria. |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Sorci L, Martynowski D, Rodionov DA, Eyobo Y, Zogaj X, Klose KE, Nikolaev EV, Magni G, Zhang H, Osterman AL. Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3083-8. doi: 10.1073/pnas.0811718106. Epub 2009 Feb 9. PMID: 19204287; PMCID: PMC2651253. | ||
Latest revision as of 05:36, 29 September 2024
nadM: bifunctional nicotinamide-nucleotide adenylyltransferase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. NadM encoded by nadM catalyze the adenylylation of the nicotinic acid mononucleotide precursor to nicotinic acid dinucleotide followed by its amidation to NAD+. Given the properties of this enzyme, we decided to use NadM to increase the total amount of NAD+ within the engineered bacteria.
Source
Francisella tularensis strain Schu S4
References
Sorci L, Martynowski D, Rodionov DA, Eyobo Y, Zogaj X, Klose KE, Nikolaev EV, Magni G, Zhang H, Osterman AL. Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3083-8. doi: 10.1073/pnas.0811718106. Epub 2009 Feb 9. PMID: 19204287; PMCID: PMC2651253.