Difference between revisions of "Part:BBa K5291028:Design"

 
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===Design Notes===
 
===Design Notes===
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We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis.
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We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. NadM encoded by nadM catalyze the adenylylation of the nicotinic acid mononucleotide precursor to nicotinic acid dinucleotide followed by its amidation to NAD+. Given the properties of this enzyme, we decided to use NadM to increase the total amount of NAD+ within the engineered bacteria.
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===Source===
 
===Source===

Revision as of 05:35, 29 September 2024


nadM: bifunctional nicotinamide-nucleotide adenylyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use it primarily to consider the role of nadM in the de novo and salvage pathways of NAD+ biosynthesis. NadM encoded by nadM catalyze the adenylylation of the nicotinic acid mononucleotide precursor to nicotinic acid dinucleotide followed by its amidation to NAD+. Given the properties of this enzyme, we decided to use NadM to increase the total amount of NAD+ within the engineered bacteria.

Source

Francisella tularensis strain Schu S4

References