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Spycatcher-VDAL-CPPs is a plant immune-activating protein engineered to contain a Spycatcher tag and an R9 cell-penetrating peptide. It can penetrate plant cell membranes and induce an ETI response intracellularly.During the experiment, we successfully constructed the fusion protein and verified its function at the molecular level. Additionally, we confirmed its immunomodulatory activity using DAB-based reactive oxygen species staining.
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SpyCatcher-VDAL-CPPs is a plant immune-activating protein engineered to contain a Spycatcher tag and an R9 cell-penetrating peptide. It can penetrate plant cell membranes and induce an ETI response intracellularly.During the experiment, we successfully constructed the fusion protein and verified its function at the molecular level. Additionally, we confirmed its immunomodulatory activity using DAB-based reactive oxygen species staining.
 
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Revision as of 04:23, 29 September 2024


SpyCatcher-VDAL-CPPs (R9-Tag)

SpyCatcher-VDAL-CPPs is a plant immune-activating protein engineered to contain a Spycatcher tag and an R9 cell-penetrating peptide. It can penetrate plant cell membranes and induce an ETI response intracellularly.During the experiment, we successfully constructed the fusion protein and verified its function at the molecular level. Additionally, we confirmed its immunomodulatory activity using DAB-based reactive oxygen species staining. 无标题文档


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]