Difference between revisions of "Part:BBa K5269027"
Line 3: | Line 3: | ||
<partinfo>BBa_K5269027 short</partinfo> | <partinfo>BBa_K5269027 short</partinfo> | ||
− | This sequence encodes INPNC and SpyCatcher. This enables surface display on the cell membrane utilizing the | + | This sequence encodes INPNC and SpyCatcher. This enables surface display on the cell membrane utilizing the SpyCatcher-SpyTag system. Also, This allows the protein to act stably on the Membrane Vesicle, allowing for efficient shRNA introduction and the ability to remain on the plant. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 03:51, 29 September 2024
INPNC-SpyCatcher
This sequence encodes INPNC and SpyCatcher. This enables surface display on the cell membrane utilizing the SpyCatcher-SpyTag system. Also, This allows the protein to act stably on the Membrane Vesicle, allowing for efficient shRNA introduction and the ability to remain on the plant.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 481
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 405
Illegal AgeI site found at 429 - 1000COMPATIBLE WITH RFC[1000]