Difference between revisions of "Part:BBa K5115043"
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__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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This composite part combines [https://parts.igem.org/Part:BBa_K51150036 BBa_K51150036(ribozyme+RBS+hpn+stem-loop)],[https://parts.igem.org/Part:BBa_K51150033 BBa_K51150033(ribozyme+RBS+RcnR_C35L+stem-loop)] and [https://parts.igem.org/Part:BBa_K51150035 BBa_K51150035(ribozyme+RBS+MTA+stem-loop)] in our ribozyme-assisted polycistronic co-expression system：pRAP. To get more information about pRAP, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM ].
  
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===Usage and Biology===
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MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can
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sequester metals that accumulate internally to reduce nickel's toxity to the 'E.coli'. RcnR_C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate.
  
 
===Characterization===
 
===Characterization===

Revision as of 03:12, 29 September 2024


ribozyme connected: Hpn, RcnR_C35L, MTA

contributed by Fudan iGEM 2023

Introduction

This composite part combines BBa_K51150036(ribozyme+RBS+hpn+stem-loop),BBa_K51150033(ribozyme+RBS+RcnR_C35L+stem-loop) and BBa_K51150035(ribozyme+RBS+MTA+stem-loop) in our ribozyme-assisted polycistronic co-expression system：pRAP. To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .

MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the 'E.coli'. RcnR_C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 875
  • 1000
    COMPATIBLE WITH RFC[1000]


References