Difference between revisions of "Part:BBa K5103001"
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===Profile=== | ===Profile=== | ||
− | Name: | + | Name: PCG004 with Cry8Da |
− | Base Pairs: | + | Base Pairs: 12261 |
− | Origin: | + | Origin: Add |
− | Properties: | + | Properties: Add |
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 01:37, 29 September 2024
PCG004 with Cry8Da
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 595
Illegal EcoRI site found at 6398
Illegal EcoRI site found at 8948
Illegal EcoRI site found at 9027
Illegal EcoRI site found at 10439
Illegal EcoRI site found at 11102
Illegal XbaI site found at 11533
Illegal PstI site found at 8079
Illegal PstI site found at 9520 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 595
Illegal EcoRI site found at 6398
Illegal EcoRI site found at 8948
Illegal EcoRI site found at 9027
Illegal EcoRI site found at 10439
Illegal EcoRI site found at 11102
Illegal NheI site found at 6234
Illegal PstI site found at 8079
Illegal PstI site found at 9520 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 595
Illegal EcoRI site found at 6398
Illegal EcoRI site found at 8948
Illegal EcoRI site found at 9027
Illegal EcoRI site found at 10439
Illegal EcoRI site found at 11102
Illegal BglII site found at 66
Illegal BglII site found at 3147
Illegal XhoI site found at 3151
Illegal XhoI site found at 11410 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 595
Illegal EcoRI site found at 6398
Illegal EcoRI site found at 8948
Illegal EcoRI site found at 9027
Illegal EcoRI site found at 10439
Illegal EcoRI site found at 11102
Illegal XbaI site found at 11533
Illegal PstI site found at 8079
Illegal PstI site found at 9520 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 595
Illegal EcoRI site found at 6398
Illegal EcoRI site found at 8948
Illegal EcoRI site found at 9027
Illegal EcoRI site found at 10439
Illegal EcoRI site found at 11102
Illegal XbaI site found at 11533
Illegal PstI site found at 8079
Illegal PstI site found at 9520
Illegal AgeI site found at 9294
Illegal AgeI site found at 10176 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 11524
Illegal BsaI.rc site found at 7949
Illegal BsaI.rc site found at 7962
Illegal BsaI.rc site found at 11537
Illegal SapI.rc site found at 1219
Illegal SapI.rc site found at 5124
Profile
Name: PCG004 with Cry8Da Base Pairs: 12261 Origin: Add Properties: Add
Usage and Biology
It includes features for selective gene expression in a dropout region that normally contains green fluorescent protein (GFP) flanked by BsaI sites. In this case we replaced GFP with Cry8Da crystal toxin sequences from Bacillus thuringiensis (Bt). Upstream, a lacI sequence encodes the lac repressor and a lac operator (lacO) overlaps with the pGrac promoter. Using this plasmid allows us to induce the expression of Cry8Da when we want using IPTG. This allows transcription of Cry8Da by causing a conformational change in Lac, releasing it from lacO.
Figure 1 . Streak plate of PCG-Cry8Da transformant colonies grown on ampicillin selective media.
Figure 2. PCG-CRY plasmid components were developed using SnapGene.