Difference between revisions of "Part:BBa K5241001"
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<span style="font-size: 150%; font-weight: bold;">Reference documentation</span>
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[1] ChiaraScanferla EnricoCaruso Viviana TeresaOrlandi FabrizioBolognese. Bacterial melanin production by heterologous expression of 4‑hydroxyphenylpyruvate dioxygenase from Pseudomonas aeruginosa[J]. International Journal of Biological Macromolecules, 2019, 133: 1072-1080.
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[2] Zhang, Qihao, et al. "Pyomelanin Production via Heterologous Expression of 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) and Construction of HPPD Inhibitor Screening Model." Journal of Bioscience and Bioengineering, vol. 136, no. 2, 2023, pp. 123-131. https://doi.org/10.1016/j.jbiosc.2023.01.003
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Revision as of 00:53, 29 September 2024


Pahdd

Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.

Gene Circuit Diagram:

Source: Pseudomonas aeruginosa

Result：

Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm

Reference documentation

[1] ChiaraScanferla EnricoCaruso Viviana TeresaOrlandi FabrizioBolognese. Bacterial melanin production by heterologous expression of 4‑hydroxyphenylpyruvate dioxygenase from Pseudomonas aeruginosa[J]. International Journal of Biological Macromolecules, 2019, 133: 1072-1080.

[2] Zhang, Qihao, et al. "Pyomelanin Production via Heterologous Expression of 4-Hydroxyphenylpyruvate Dioxygenase (HPPD) and Construction of HPPD Inhibitor Screening Model." Journal of Bioscience and Bioengineering, vol. 136, no. 2, 2023, pp. 123-131. https://doi.org/10.1016/j.jbiosc.2023.01.003


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal BglII site found at 936
    Illegal XhoI site found at 869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 1000
    COMPATIBLE WITH RFC[1000]