Difference between revisions of "Part:BBa K5477035"

 
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<partinfo>BBa_K5477035 short</partinfo>
 
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UDPD-pGAL1/10-UGT2B15 is a composite part and is one of our detoxification modules. In our system, UGT2B15 and UDPD were combined to create a highly efficient detoxification module capable of neutralizing environmental toxins such as bisphenol A (BPA). UGT2B15 is responsible for the glucuronidation of these toxic compounds, a process in which glucuronic acid is added to the toxins, rendering them more water-soluble and easier to excrete from the body. However, for UGT2B15 to perform this function effectively, it requires a steady supply of UDP-glucuronic acid, the donor molecule of glucuronic acid used in this reaction. This is where UDPD (UDP-glucose dehydrogenase) becomes essential. UDPD catalyzes the conversion of UDP-glucose to UDP-glucuronic acid, ensuring that there is an ample supply of this critical substrate for UGT2B15.
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UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, which plays a role in the phase II metabolism of xenobiotics and endogenous compounds. UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body. This detoxification process is essential for eliminating harmful substances such as drugs, environmental toxins, and metabolic byproducts. In particular, UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals. By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity. The enzyme is expressed predominantly in the liver but is also found in other tissues such as the kidney, prostate, and gastrointestinal tract, where it participates in localized detoxification processes (1).
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By incorporating UGT2B15 into the detoxification system, the goal is to metabolize BPA through glucuronidation, rendering it less harmful and easier to excrete from the cell or organism. In our system, UGT2B15 was integrated in the following composites together with UDPD.
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<partinfo>BBa_K5477035 parameters</partinfo>
 
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===References===
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1. Ramírez, Viviana & Gálvez Ontiveros, Yolanda & Porras, Patricia & Martinez-Gonzalez, Luis & Rivas, Ana & Alvarez-Cubero, María. (2021). METABOLIC pathways, alterations in MIRNAS expression and effects of genetic polymorphisms of bisphenol a analogues: A SYSTEMATIC review. Environmental Research. 197. 111062. 10.1016/j.envres.2021.111062.

Revision as of 20:13, 28 September 2024


UDPD-pGAL1/10-UGT2B15 detox module against BPA


UGT2B15 (UDP-glucuronosyltransferase 2B15) is an enzyme belonging to the UDP-glucuronosyltransferase (UGT) family, which plays a role in the phase II metabolism of xenobiotics and endogenous compounds. UGT2B15 is primarily involved in the process of glucuronidation, a biochemical reaction where glucuronic acid is transferred to lipophilic molecules, making them more water-soluble and easier to excrete from the body. This detoxification process is essential for eliminating harmful substances such as drugs, environmental toxins, and metabolic byproducts. In particular, UGT2B15 is responsible for the glucuronidation of a wide range of compounds, including bisphenol A (BPA), steroid hormones (like androgens), and pharmaceuticals. By conjugating these substances with glucuronic acid, UGT2B15 facilitates their removal via urine or bile, reducing their biological activity and toxicity. The enzyme is expressed predominantly in the liver but is also found in other tissues such as the kidney, prostate, and gastrointestinal tract, where it participates in localized detoxification processes (1).

By incorporating UGT2B15 into the detoxification system, the goal is to metabolize BPA through glucuronidation, rendering it less harmful and easier to excrete from the cell or organism. In our system, UGT2B15 was integrated in the following composites together with UDPD.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3466
    Illegal PstI site found at 542
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3466
    Illegal PstI site found at 542
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3466
    Illegal BglII site found at 1122
    Illegal BamHI site found at 3286
    Illegal BamHI site found at 3580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3466
    Illegal PstI site found at 542
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3466
    Illegal PstI site found at 542
    Illegal AgeI site found at 1828
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Ramírez, Viviana & Gálvez Ontiveros, Yolanda & Porras, Patricia & Martinez-Gonzalez, Luis & Rivas, Ana & Alvarez-Cubero, María. (2021). METABOLIC pathways, alterations in MIRNAS expression and effects of genetic polymorphisms of bisphenol a analogues: A SYSTEMATIC review. Environmental Research. 197. 111062. 10.1016/j.envres.2021.111062.