Difference between revisions of "Part:BBa K079032"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K079032 short</partinfo> | <partinfo>BBa_K079032 short</partinfo> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K079032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K079032 SequenceAndFeatures</partinfo> | ||
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| + | We experimentally observed that bacterial growth curve can change if cells are transformed with different genetic circuits. Indeed, we observed that cells trasformed with BBa_K079031 and with BBa_K079032 display different growth. Moreover, we reasoned that is not sufficient to characterized a part or a device in terms of fluorescence, but is also important to understand how the bacterial cells grow and how the fluorescence expressed is related to the growth phase. For this reason, we analized both the growth and fluorescence curves in time and used them to experimentally characterize our parts and fill the experience section. | ||
Revision as of 20:43, 21 October 2009
GFP reporter protein under the control of the BBa_J23100 constitutive promoter
GFP reporter protein under the control of the BBa_J23100 costitutive promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
We experimentally observed that bacterial growth curve can change if cells are transformed with different genetic circuits. Indeed, we observed that cells trasformed with BBa_K079031 and with BBa_K079032 display different growth. Moreover, we reasoned that is not sufficient to characterized a part or a device in terms of fluorescence, but is also important to understand how the bacterial cells grow and how the fluorescence expressed is related to the growth phase. For this reason, we analized both the growth and fluorescence curves in time and used them to experimentally characterize our parts and fill the experience section.