Difference between revisions of "Part:BBa K180002"

 
 
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<partinfo>BBa_K180002 short</partinfo>
 
<partinfo>BBa_K180002 short</partinfo>
  
 
PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.
 
PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.
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The lacIq sequence preceding the tac promoter ensures that tac pR is in the 'OFF' state naturally. Green fluorescent protein is produced if IPTG successfully induces the promoter, demonstrating the regulatory effect of Lac upon the promoter.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:42, 21 October 2009

tac pR testing plasmid (GFP)

PTAC promoter followed by an RBS, green fluorescent protein and stop codon, for testing the effectiveness of the PTAC hybrid promoter designed by iGEM2009 Team:Southampton.

The lacIq sequence preceding the tac promoter ensures that tac pR is in the 'OFF' state naturally. Green fluorescent protein is produced if IPTG successfully induces the promoter, demonstrating the regulatory effect of Lac upon the promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2117