Difference between revisions of "Part:BBa K190015:Experience"
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Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1). | Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1). | ||
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:Figure 1: RFP fluorescence increasing upon RFP expression under pArsR promoter (K190015) | :Figure 1: RFP fluorescence increasing upon RFP expression under pArsR promoter (K190015) | ||
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Revision as of 20:42, 21 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K190015
BBa_J33201 (Available) E. coli chromosomal ars promoter with arsR repressor gene
BBa_J33203 (Available) E. coli ars promoter, arsR gene and lacZ
BBa_K190023 (Planning) E. coli ars promoter, with RBS site
BBa_K190033 (Planning) E. coli ars promoter, GVP gene cluster
User Reviews
UNIQ781c3036c1b635ed-partinfo-00000000-QINU
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iGEM Groningen 2009 |
The part (BBa_K190015) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression. |
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iGEM Groningen 2009 |
Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1).
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UNIQ781c3036c1b635ed-partinfo-00000004-QINU