Difference between revisions of "Part:BBa K5107002"

 
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<partinfo>BBa_K5107002 short</partinfo>
 
<partinfo>BBa_K5107002 short</partinfo>
  
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HRE5 is the 5 times repeats of th HRE minimal to increase the interaction surface of the hormone receptor with the DNA.
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HRE5 is the five tandem repeats of the HRE minimal to increase the interaction surface of the hormone receptor with the DNA.
  
  
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===Usage and Biology===
 
===Usage and Biology===
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Previously, in-cell systems have successfully used five tandem repeats of the REs for different human hormonal receptors[1].
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Inserted in plasmids such as those we used for our in cell-assay, it is shown efficient to express Lac-Z when the associated hormone-receptor complex is present in the cell. In this manner, hormone work as trancription activator.
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<center><html><img src="https://static.igem.wiki/teams/5107/eng-incellsystem.webp"width="300" height="300"><html></center>
  
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<html><p><b>Figure 1 | </b>Schematic overview of the in cell system which uses the Lac-Z as reporter gene when hormone/edc i spresent in the cell.</p></html>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5107002 SequenceAndFeatures</partinfo>
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===Sequence and Features===
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<partinfo>BBa_K5107000 SequenceandFeatures</partinfo>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5107002 parameters</partinfo>
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<partinfo>BBa_K5107000 parameters</partinfo>
 
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===References===
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1.Miller, C. A., Tan, X., Wilson, M., Bhattacharyya, S., & Ludwig, S. (2010). Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid, 63(2), 73–78. https://doi.org/10.1016/j.plasmid.2009.11.003
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2.Tan, M. E., Li, J., Xu, H. E., Melcher, K., & Yong, E. (2014). Androgen receptor: structure, role in prostate cancer and drug discovery. Acta Pharmacologica Sinica, 36(1), 3–23. https://doi.org/10.1038/aps.2014.18

Revision as of 19:21, 28 September 2024


HRE5


HRE5 is the five tandem repeats of the HRE minimal to increase the interaction surface of the hormone receptor with the DNA.


Usage and Biology

Previously, in-cell systems have successfully used five tandem repeats of the REs for different human hormonal receptors[1]. Inserted in plasmids such as those we used for our in cell-assay, it is shown efficient to express Lac-Z when the associated hormone-receptor complex is present in the cell. In this manner, hormone work as trancription activator.

Figure 1 | Schematic overview of the in cell system which uses the Lac-Z as reporter gene when hormone/edc i spresent in the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1.Miller, C. A., Tan, X., Wilson, M., Bhattacharyya, S., & Ludwig, S. (2010). Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid, 63(2), 73–78. https://doi.org/10.1016/j.plasmid.2009.11.003

‌

2.Tan, M. E., Li, J., Xu, H. E., Melcher, K., & Yong, E. (2014). Androgen receptor: structure, role in prostate cancer and drug discovery. Acta Pharmacologica Sinica, 36(1), 3–23. https://doi.org/10.1038/aps.2014.18