Difference between revisions of "Part:BBa K243027"
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===Usage and Biology===
 
===Usage and Biology===
  
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Plasmids encoding the sequences for the bZip domains of c-Fos linked to a His-tag allow the purification which could then be expressed as soluble proteins in Escherichia coli.<br>
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Plasmids encoding the sequences for the bZip domains of c-Fos linked to a His-tag allow the purification over a Ni-NTA column and the expression as soluble proteins in Escherichia coli.<br>
  
  

Revision as of 20:26, 21 October 2009

His-FOS

Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers and binds DNA as a dimer. Two classes of core DNA sequences, the sequences TRE (TGACTCA) and CRE (TGACGTCA), can be recognized by the AP-1. Nine homologues of the AP-1 leucine zipper region have been characterized. All of them are able to form heterodimers, some also form homodimers. One of them is the so called c-Fos. Via leucin zipper they interact among each other and with their bipartite domain they bind DNA. (Abate et al., Mol Cell Biol. 1991 July).



1fos bio r 250.jpg Heterodimeric Fos complex (http://www.rcsb.org/pdb/explore/images.do?structureId=1FOS)


Usage and Biology

Plasmids encoding the sequences for the bZip domains of c-Fos linked to a His-tag allow the purification over a Ni-NTA column and the expression as soluble proteins in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]