Difference between revisions of "Part:BBa K243012"

Revision as of 20:11, 21 October 2009

Strep-DigA-Middle Linker-Fok_i

This part unites some of our basic parts. The combination of a StrepTag, an Anticalin-Tag and the linked protein domain Fok_i makes it possible to purify and detect the function of the protein. The DigoxigeninA tag allows the coupling to an digoxigenin linked oligonucleotide.

Usage and Biology

This composite part is one part of our universal endonuclease and needs another composite part like BBa_K243024 to build a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an Digoxigenin labeled oligonucleotide. The middle linker creates a distance of 24bp between the DigA and the linked Fok_i protein domain. The StrepTag serves as purification tag for Streptavidin column purification.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 278
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K243012 short</partinfo>
-This part unites some of our basic parts. The combination of a [https://parts.igem.org/Part:BBa_K157012 StrepTag], a [https://parts.igem.org/Part:BBa_K243003 Anticalin-Tag] and the linked protein domain [https://parts.igem.org/Part:BBa_K243001 Fok_i], makes it possible to purifier and detect the function of the protein. The DigoxigeninA tag allows  the coupling to an digoxigenin linked oligonucleotide.
+This part unites some of our basic parts. The combination of a [https://parts.igem.org/Part:BBa_K157012 StrepTag], an [https://parts.igem.org/Part:BBa_K243003 Anticalin-Tag] and the linked protein domain [https://parts.igem.org/Part:BBa_K243001 Fok_i] makes it possible to purify and detect the function of the protein. The DigoxigeninA tag allows the coupling to an digoxigenin linked oligonucleotide.
 ===Usage and Biology===
-This composite part is one part of our universal endonuclease and it needs another composite part like [https://parts.igem.org/Part:BBa_K243024 BBa_K243024] to built to a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an Digoxigenin labeled oligonucleotide. The middle linker mades a distance from 24bp between the DigA and the linked Fok_i protein domain. The StrepTag serve as purification tag for Streptavidin column purification.
+This composite part is one part of our universal endonuclease and needs another composite part like [https://parts.igem.org/Part:BBa_K243024 BBa_K243024] to build a functional heterodimer. The DigA tag leads the part to DNA which is hybridized with an Digoxigenin labeled oligonucleotide. The middle linker creates a distance of 24bp between the DigA and the linked Fok_i protein domain. The StrepTag serves as purification tag for Streptavidin column purification.