Difference between revisions of "Part:BBa K5115062"
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===Introduction===
 
===Introduction===
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This composite part is composed of hoxU-GS-EP and improved ribozyme-assisted polycistronic co-expression system: pRAP. Get details about hoxU in [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)] and EP in [https://parts.igem.org/Part:BBa_K5115002 BBa_K5115002(EP)].
  
 
===Usage and Biology===
 
===Usage and Biology===
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Binding hoxU and EP can tether hydrogenase genes to the α-carboxysome, providing a protective microenvironment for the hydrogenase enzyme, enhancing its catalytic activity and stability. The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
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===Characterization===
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Revision as of 14:45, 28 September 2024


ribozyme+RBS+hoxU-GS-EP+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of hoxU-GS-EP and improved ribozyme-assisted polycistronic co-expression system: pRAP. Get details about hoxU in BBa_K5115020(hox and hyp operon) and EP in BBa_K5115002(EP).

Usage and Biology

Binding hoxU and EP can tether hydrogenase genes to the α-carboxysome, providing a protective microenvironment for the hydrogenase enzyme, enhancing its catalytic activity and stability. The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 485
    Illegal BglII site found at 563
    Illegal XhoI site found at 493
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 446
    Illegal AgeI site found at 1445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 267
    Illegal BsaI site found at 429
    Illegal SapI.rc site found at 378


References