Difference between revisions of "Part:BBa K5097009"

Revision as of 14:01, 28 September 2024

SpeA (arginine decarboxylase)

Usage and Biology

This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al.,1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1389
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1167
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5097009 short</partinfo>
-This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase. This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine. The 2024 iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.
+===Usage and Biology===
+This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al.,1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.
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-===Usage and Biology===
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