Difference between revisions of "Part:BBa K5115054"
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===Introduction=== | ===Introduction=== | ||
| + | The staygold is a green fluorescent protein (GFP) derived from the jellyfish ''Cytaeis uchidae''. It has high brightness, high stability and low phototoxity,<ref>Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., & Miyawaki, A. (2022). A highly photostable and bright green fluorescent protein. Nature Biotechnology, 40(7), 1132–1142.</ref> which is a perfect choice for our design. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | There actually exists a stayGold part in registry: [https://parts.igem.org/Part:BBa_K4491134 BBa_K4491134]. We add a Gly and a Ser to it as a linker, allowing it to link with EP. | |
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Latest revision as of 13:06, 28 September 2024
stayGold-GS
Introduction
The staygold is a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. It has high brightness, high stability and low phototoxity,[1] which is a perfect choice for our design.
Usage and Biology
There actually exists a stayGold part in registry: BBa_K4491134. We add a Gly and a Ser to it as a linker, allowing it to link with EP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 638
Illegal BsaI.rc site found at 658
References
- ↑ Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., & Miyawaki, A. (2022). A highly photostable and bright green fluorescent protein. Nature Biotechnology, 40(7), 1132–1142.