Difference between revisions of "Part:BBa K5115054"

 
 
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<partinfo>BBa_K5115054 short</partinfo>
 
<partinfo>BBa_K5115054 short</partinfo>
  
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/5115/czh/mineral-logo.svg" alt="contributed by Fudan iGEM 2023"></html>
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===Introduction===
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The staygold is a green fluorescent protein (GFP) derived from the jellyfish ''Cytaeis uchidae''. It has high brightness, high stability and low phototoxity,<ref>Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., & Miyawaki, A. (2022). A highly photostable and bright green fluorescent protein. Nature Biotechnology, 40(7), 1132–1142.</ref> which is a perfect choice for our design.
  
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===Usage and Biology===
 
===Usage and Biology===
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There actually exists a stayGold part in registry:  [https://parts.igem.org/Part:BBa_K4491134 BBa_K4491134]. We add a Gly and a Ser to it as a linker, allowing it to link with EP.
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<partinfo>BBa_K5115054 parameters</partinfo>
 
<partinfo>BBa_K5115054 parameters</partinfo>
 
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==References==
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<references />

Latest revision as of 13:06, 28 September 2024


stayGold-GS

contributed by Fudan iGEM 2023

Introduction

The staygold is a green fluorescent protein (GFP) derived from the jellyfish Cytaeis uchidae. It has high brightness, high stability and low phototoxity,[1] which is a perfect choice for our design.

Usage and Biology

There actually exists a stayGold part in registry: BBa_K4491134. We add a Gly and a Ser to it as a linker, allowing it to link with EP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 638
    Illegal BsaI.rc site found at 658


References

  1. ↑ Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., & Miyawaki, A. (2022). A highly photostable and bright green fluorescent protein. Nature Biotechnology, 40(7), 1132–1142.