Difference between revisions of "Part:BBa K245101:Design"

 
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<partinfo>BBa_K245101 short</partinfo>
 
<partinfo>BBa_K245101 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.  Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV,  thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.
 
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===Source===
 
===Source===
  
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Composed from LL37-ELST-GCN-ELST intermediate part and P1 basic part  according to assembly standard 37.
  
 
===References===
 
===References===

Latest revision as of 19:56, 21 October 2009

LL37-ELST-GCN-ELST-P1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV, thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.

Source

Composed from LL37-ELST-GCN-ELST intermediate part and P1 basic part according to assembly standard 37.

References