Difference between revisions of "Part:BBa K245051"
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<partinfo>BBa_K245051 short</partinfo> | <partinfo>BBa_K245051 short</partinfo> | ||
| − | + | This part is made of two domains – GyrB, a 24 kDa fragment of gyrase subunit B, and CutA1, a copper binding protein. It is one of the constructs whose assembly can be regulated. When an aminocoumarin antibiotic called coumermycin is added, GyrB domains dimerize. Because this is a combination of dimerization and trimerization domains, the resulting structure is a lattice with hexagonal pores. Another aminocoumarin antibiotic novobiocin inhibits this structure. This part is similar to GyrB-CutA1 (BBa_K245050) but has extended linker according to Standard 37. | |
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Latest revision as of 19:46, 21 October 2009
GyrB-SG-SG-CutA1
This part is made of two domains – GyrB, a 24 kDa fragment of gyrase subunit B, and CutA1, a copper binding protein. It is one of the constructs whose assembly can be regulated. When an aminocoumarin antibiotic called coumermycin is added, GyrB domains dimerize. Because this is a combination of dimerization and trimerization domains, the resulting structure is a lattice with hexagonal pores. Another aminocoumarin antibiotic novobiocin inhibits this structure. This part is similar to GyrB-CutA1 (BBa_K245050) but has extended linker according to Standard 37.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 428
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 100
Illegal AgeI site found at 238 - 1000COMPATIBLE WITH RFC[1000]