Difference between revisions of "Part:BBa K243022"
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<partinfo>BBa_K243022 short</partinfo>
 
<partinfo>BBa_K243022 short</partinfo>
  
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This combination uses the benefits of an Streptavidin-tag for purification. It is also linked with a DigoxygenineA-tag. The Long Linker (GlySerGlyGly)x3 connects the parts and adds additional space between them to guarantee the independent function of FluA-tag and the protein domain Fok_i.The DsbA tag is used to transfer the produced protein into the periplasm. This makes is easier for us to extract the protein.
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This combination uses the benefits of an Streptavidin-tag for purification. It is also linked with a DigoxygenineA-tag       [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243003 DigA]. The Split Linker connects the parts and adds additional space between them to guarantee the independent function of DigA-tag and the protein domain Fok_a.The DsbA tag is used to transfer the produced protein into the periplasm.  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Revision as of 19:36, 21 October 2009

DsbA-Strep-DigA-Split Linker-Fok_a

This combination uses the benefits of an Streptavidin-tag for purification. It is also linked with a DigoxygenineA-tag DigA. The Split Linker connects the parts and adds additional space between them to guarantee the independent function of DigA-tag and the protein domain Fok_a.The DsbA tag is used to transfer the produced protein into the periplasm.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1186