Difference between revisions of "Part:BBa K5291011:Design"
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===Source=== | ===Source=== | ||
| − | The plasmid R1 from Escherichia coli | + | The plasmid R1 from <i>Escherichia coli</i> |
===References=== | ===References=== | ||
| − | [1]Gerdes, K. and E.G. Wagner, RNA antitoxins. Curr Opin Microbiol, 2007. 10(2): p. 117-24. | + | [1]Gerdes, K. and E.G. Wagner, RNA antitoxins. Curr Opin Microbiol, 2007. 10(2): p. 117-24.<br> |
| − | [2]Thisted, T. and K. Gerdes, Mechanism of post-segregational killing by the hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene. Journal of Molecular Biology, 1992. 223(1): p. 41-54. | + | [2]Thisted, T. and K. Gerdes, Mechanism of post-segregational killing by the hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene. Journal of Molecular Biology, 1992. 223(1): p. 41-54.<br> |
| − | [3]Franch, T., A.P. Gultyaev and K. Gerdes, Programmed cell death by hok/sok of plasmid R1: Processing at the hok mRNA 3′-end triggers structural rearrangements that allow translation and antisense RNA binding11Edited by D. E. Draper. Journal of Molecular Biology, 1997. 273(1): p. 38-51. | + | [3]Franch, T., A.P. Gultyaev and K. Gerdes, Programmed cell death by hok/sok of plasmid R1: Processing at the hok mRNA 3′-end triggers structural rearrangements that allow translation and antisense RNA binding11Edited by D. E. Draper. Journal of Molecular Biology, 1997. 273(1): p. 38-51.<br> |
[4]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703. | [4]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703. | ||
Latest revision as of 03:50, 28 September 2024
sok
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene is from the hok/sok system, which is used as the repressor of the hok mRNA. We replace its promoter with a strong promoter that can be activated by citrate, making sok a gene express when there is citrate in the environment to secure the living of cells. The gene sok is also a component of the plasmid anti-loss system.
Source
The plasmid R1 from Escherichia coli
References
[1]Gerdes, K. and E.G. Wagner, RNA antitoxins. Curr Opin Microbiol, 2007. 10(2): p. 117-24.
[2]Thisted, T. and K. Gerdes, Mechanism of post-segregational killing by the hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene. Journal of Molecular Biology, 1992. 223(1): p. 41-54.
[3]Franch, T., A.P. Gultyaev and K. Gerdes, Programmed cell death by hok/sok of plasmid R1: Processing at the hok mRNA 3′-end triggers structural rearrangements that allow translation and antisense RNA binding11Edited by D. E. Draper. Journal of Molecular Biology, 1997. 273(1): p. 38-51.
[4]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.