Difference between revisions of "Part:BBa K5291011:Design"

 
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===Design Notes===
 
===Design Notes===
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None
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The gene is from the <i>hok</i>/<i>sok</i> system, which is used as the repressor of the <i>hok</i> mRNA. We replace its promoter with a strong promoter that can be activated by citrate, making <i>sok</i> a gene express when there is citrate in the environment to secure the living of cells. The gene <i>sok</i> is also a component of the plasmid anti-loss system.
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===Source===
 
===Source===
  
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The plasmid R1 from Escherichia coli
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The plasmid R1 from <i>Escherichia coli</i>
  
 
===References===
 
===References===
 +
[1]Gerdes, K. and E.G. Wagner, RNA antitoxins. Curr Opin Microbiol, 2007. 10(2): p. 117-24.<br>
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[2]Thisted, T. and K. Gerdes, Mechanism of post-segregational killing by the hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene. Journal of Molecular Biology, 1992. 223(1): p. 41-54.<br>
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[3]Franch, T., A.P. Gultyaev and K. Gerdes, Programmed cell death by hok/sok of plasmid R1: Processing at the hok mRNA 3′-end triggers structural rearrangements that allow translation and antisense RNA binding11Edited by D. E. Draper. Journal of Molecular Biology, 1997. 273(1): p. 38-51.<br>
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[4]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.

Latest revision as of 03:50, 28 September 2024


sok


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene is from the hok/sok system, which is used as the repressor of the hok mRNA. We replace its promoter with a strong promoter that can be activated by citrate, making sok a gene express when there is citrate in the environment to secure the living of cells. The gene sok is also a component of the plasmid anti-loss system.

Source

The plasmid R1 from Escherichia coli

References

[1]Gerdes, K. and E.G. Wagner, RNA antitoxins. Curr Opin Microbiol, 2007. 10(2): p. 117-24.
[2]Thisted, T. and K. Gerdes, Mechanism of post-segregational killing by the hok/sok system of plasmid R1: Sok antisense RNA regulates hok gene expression indirectly through the overlapping mok gene. Journal of Molecular Biology, 1992. 223(1): p. 41-54.
[3]Franch, T., A.P. Gultyaev and K. Gerdes, Programmed cell death by hok/sok of plasmid R1: Processing at the hok mRNA 3′-end triggers structural rearrangements that allow translation and antisense RNA binding11Edited by D. E. Draper. Journal of Molecular Biology, 1997. 273(1): p. 38-51.
[4]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.