Difference between revisions of "Part:BBa K5291010:Design"

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===Design Notes===
 
===Design Notes===
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In our design we choose a strong promoter that can be activated by citrate. The hok/sok system is a efficient system controlled by two promoters with different strength, which fits right in with our design with a strong promoter and a weak promoter.
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The <i>hok</i>/<i>sok</i> system is a efficient system controlled by two promoters with different strength to realize plasmid anti-loss as well as cell suicide, which fits right in with our design with a strong promoter and a weak promoter.
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===Source===
 
===Source===
  
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Escherichia coli
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The plasmid R1 from <i>Escherichia coli</i>
  
 
===References===
 
===References===
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[1]Gerdes, K., et al., Mechanism of postsegregational killing by the hok gene product of the parB system of plasmid R1 and its homology with the relF gene product of the E. coli relB operon. The EMBO Journal, 1986. 5(8): p. 2023-2029.
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[1]Gerdes, K., et al., Mechanism of postsegregational killing by the hok gene product of the parB system of plasmid R1 and its homology with the relF gene product of the E. coli relB operon. The EMBO Journal, 1986. 5(8): p. 2023-2029.<br>
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[2]Gerdes, K., et al., The hok killer gene family in gram-negative bacteria. The New biologist, 1990. 2: p. 946-56.
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[2]Gerdes, K., et al., The hok killer gene family in gram-negative bacteria. The New biologist, 1990. 2: p. 946-56.<br>
 
[3]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.
 
[3]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.

Latest revision as of 03:44, 28 September 2024


hok/mok


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The hok/sok system is a efficient system controlled by two promoters with different strength to realize plasmid anti-loss as well as cell suicide, which fits right in with our design with a strong promoter and a weak promoter.

Source

The plasmid R1 from Escherichia coli

References

[1]Gerdes, K., et al., Mechanism of postsegregational killing by the hok gene product of the parB system of plasmid R1 and its homology with the relF gene product of the E. coli relB operon. The EMBO Journal, 1986. 5(8): p. 2023-2029.
[2]Gerdes, K., et al., The hok killer gene family in gram-negative bacteria. The New biologist, 1990. 2: p. 946-56.
[3]Gong, C.C. and S. Klumpp, Modeling sRNA-Regulated Plasmid Maintenance. PLoS One, 2017. 12(1): p. e0169703.