Difference between revisions of "Part:BBa K5291006:Design"
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===Source=== | ===Source=== | ||
| − | Rhodopseudomonas palustris CGA009 | + | <i>Rhodopseudomonas palustris</i> CGA009 |
===References=== | ===References=== | ||
| − | [1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966. | + | [1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966.<br> |
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213. | [2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213. | ||
Latest revision as of 03:20, 28 September 2024
acaP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 223
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 223
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 152
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 223
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 223
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene acaP is from Rhodopseudomonas palustris CGA009, which ensures its normal expression in the engineered R.palistris.
Source
Rhodopseudomonas palustris CGA009
References
[1]Puskas, L.G., et al., A periplasmic, alpha-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology (Reading), 2000. 146 ( Pt 11): p. 2957-2966.
[2]Thorbecke, R., et al., The gene dosage effect of carbonic anhydrase on the biosynthesis of poly ( 3-hydroxybutyrate ) under autotrophic and mixotrophic culture conditions. POLYMER JOURNAL, 2021. 53(1): p. 209-213.