Difference between revisions of "Part:BBa K5366017"

Revision as of 02:33, 28 September 2024

AJC7

Pseudothermotoga hypogea DSM-derived sequences with tagatose-4-epimerase activity

In the present study, an unknown functional protein from Pseudothermotoga hypogea DSM, exhibiting Tagatose-4-epimerase activity, was identified through gene mining and designated as AGC7. The binding free energy of the receptor-ligand complex was calculated using a CHARMm-based energy functional along with implicit solvent methods. These free energies were estimated by minimizing the ligand energy in the presence of the receptor, employing both the steepest descent and conjugate gradient methods. Instead of utilizing the more costly molecular surface approximation, the effective Born radius was calculated using the Generalized Born Simple Switching (GBSW) implicit solvent model. This model features smooth dielectric boundaries that incorporate van der Waals surfaces. Using this approach, we calculated the free energy of binding between AGC7 and fructose (Fig. 1).

Fig. 1 Free energy of binding between AJC7 and fructose

From Figure 1, the free energy of docking between AJC7 and fructose is -9.9971.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 501
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1003
1000
COMPATIBLE WITH RFC[1000]

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+  <i><b>  Fig. 1 Free energy of binding between AJC7 and fructose<br><br></b></I>
    <div class="unterschrift"><bFig. 1 Construction of pMTL-Pfba-Bs2 recombinant plasmid</b>
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-  Fig. 1 Free energy of binding between AJC7 and fructose
 From Figure 1, the free energy of docking between AJC7 and fructose is -9.9971.