Difference between revisions of "Part:BBa K243032"
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There was a strong fluorescence signal under the fluorescence microscope after the induction of the cells with IPTG.The ''E.colis''(RV308) were excited with the wavelength 505nm and then some pictures of the fluorescencewere made.<br> | There was a strong fluorescence signal under the fluorescence microscope after the induction of the cells with IPTG.The ''E.colis''(RV308) were excited with the wavelength 505nm and then some pictures of the fluorescencewere made.<br> | ||
[[Image:Freiburg RV308 mit fluo Oligos3 (c1+c2).JPG|350x250px]] | [[Image:Freiburg RV308 mit fluo Oligos3 (c1+c2).JPG|350x250px]] | ||
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| + | The cell extract were check by SDS-page to get information about the expression of proteins.<br> | ||
| + | [[Image:SDSfokvenus.jpg]] | ||
===Purification=== | ===Purification=== | ||
Revision as of 19:12, 21 October 2009
Fok_a-Venus
This part is like part the protein domain Fok_a from our universal endonuclease, but it has an additional linked YFP-marker.
Usage and Biology
The addition of a fluorescent protein(Venus) to the protein construct, enables the detection by fluorescence microscope and new way of purification by GFP-trap column.The Venus protein was fusioned C'terminal to the protein domain, so when the protein is expressed the fluorescence signal of the Venus protein can be seen.
Detection
There was a strong fluorescence signal under the fluorescence microscope after the induction of the cells with IPTG.The E.colis(RV308) were excited with the wavelength 505nm and then some pictures of the fluorescencewere made.
.JPG)
The cell extract were check by SDS-page to get information about the expression of proteins.
Purification
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487