Difference between revisions of "Part:BBa K243032"
Line 2: Line 2:
 
<partinfo>BBa_K243032 short</partinfo>
 
<partinfo>BBa_K243032 short</partinfo>
  
−
This is like our [https://parts.igem.org/Part:BBa_K243000 protein domain Fok_a] from our universal endonuclease, but it has an additional linked YFP-marker.   
+
This part is like part the [https://parts.igem.org/Part:BBa_K243000 protein domain Fok_a] from our universal endonuclease, but it has an additional linked [https://parts.igem.org/Part:BBa_K157008 YFP-marker].   
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The addition of a fluorescent protein(Venus) to the protein construct, enables the detection by fluorescence microscope and new way of purification by GFP-trap column.The Venus protein was fusioned C'terminal to the protein domain, so when the protein is expressed the fluorescence signal of the Venus protein can be seen. 
 +
<br>
 +
===Detection===
 +
There was a strong fluorescence signal under the fluorescence microscope after the induction of the cells with IPTG.The ''E.colis''(RV308) were excited with the wavelength 505nm and then some pictures of the fluorescencewere made.<br>
 +
[[Image:Freiburg RV308 mit fluo Oligos3 (c1+c2).JPG|350x250px]]
  
−
<!-- -->
+
===Purification===
 +
 
 +
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K243032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243032 SequenceAndFeatures</partinfo>

Revision as of 19:03, 21 October 2009

Fok_a-Venus

This part is like part the protein domain Fok_a from our universal endonuclease, but it has an additional linked YFP-marker.

Usage and Biology

The addition of a fluorescent protein(Venus) to the protein construct, enables the detection by fluorescence microscope and new way of purification by GFP-trap column.The Venus protein was fusioned C'terminal to the protein domain, so when the protein is expressed the fluorescence signal of the Venus protein can be seen.

Detection

There was a strong fluorescence signal under the fluorescence microscope after the induction of the cells with IPTG.The E.colis(RV308) were excited with the wavelength 505nm and then some pictures of the fluorescencewere made.
Freiburg RV308 mit fluo Oligos3 (c1+c2).JPG

Purification

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487