Difference between revisions of "Part:BBa K5109014:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag.  
 
The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag.  
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Those sites have been designed in order to extract the Hydrolase sequence from the surface display system in which it is inserted (BBa_K5109021), in order to exchange it with other enzymes, without modifying the rest of the expression cassette.
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Those sites have been designed in order to extract the Laccase sequence from the surface display system in which it is inserted (BBa_K5109021), in order to exchange it with other enzymes, without modifying the rest of the expression cassette.
 
This part was designed for a multiple cloning experiment which involved it's exchange with three different enzymes, structured in the same way, encoded as part BBa_K5109013, BBa_K5109015, and BBa_K5109016.  
 
This part was designed for a multiple cloning experiment which involved it's exchange with three different enzymes, structured in the same way, encoded as part BBa_K5109013, BBa_K5109015, and BBa_K5109016.  
  
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===Source===
 
===Source===
  
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This part was identified with accurate bioinformatic research into Escherichia coli sp genome.
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The coding sequence of the ECOL laccase was identified inside E. coli sp genome, and on the registry is originally reported as part BBa_K863006
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The sequence of the part was then reported on Benchling and synthezised.
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===References===
 
===References===

Latest revision as of 13:53, 27 September 2024


LacE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 234
    Illegal NgoMIV site found at 455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1


Design Notes

The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag. Those sites have been designed in order to extract the Laccase sequence from the surface display system in which it is inserted (BBa_K5109021), in order to exchange it with other enzymes, without modifying the rest of the expression cassette. This part was designed for a multiple cloning experiment which involved it's exchange with three different enzymes, structured in the same way, encoded as part BBa_K5109013, BBa_K5109015, and BBa_K5109016.


Source

The coding sequence of the ECOL laccase was identified inside E. coli sp genome, and on the registry is originally reported as part BBa_K863006

References