Difference between revisions of "Part:BBa K5109014:Design"
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===Design Notes=== | ===Design Notes=== | ||
The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag. | The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag. | ||
| − | Those sites have been designed in order to extract the | + | Those sites have been designed in order to extract the Laccase sequence from the surface display system in which it is inserted (BBa_K5109021), in order to exchange it with other enzymes, without modifying the rest of the expression cassette. |
This part was designed for a multiple cloning experiment which involved it's exchange with three different enzymes, structured in the same way, encoded as part BBa_K5109013, BBa_K5109015, and BBa_K5109016. | This part was designed for a multiple cloning experiment which involved it's exchange with three different enzymes, structured in the same way, encoded as part BBa_K5109013, BBa_K5109015, and BBa_K5109016. | ||
Revision as of 13:52, 27 September 2024
LacE
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1579
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 234
Illegal NgoMIV site found at 455 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Design Notes
The enzymatic sequence has two different enzyme restriction sites at the ends: a BsaI restriction site upstream the coding sequence, and a BamHI restriction site downstream the sequence, after the His - Tag. Those sites have been designed in order to extract the Laccase sequence from the surface display system in which it is inserted (BBa_K5109021), in order to exchange it with other enzymes, without modifying the rest of the expression cassette. This part was designed for a multiple cloning experiment which involved it's exchange with three different enzymes, structured in the same way, encoded as part BBa_K5109013, BBa_K5109015, and BBa_K5109016.
Source
This part was identified with accurate bioinformatic research into Escherichia coli sp genome. The sequence of the part was then reported on Benchling and synthezised.