Difference between revisions of "Part:BBa K5034205:Design"
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Polyphosphate kinase 2(PPK2) from <i>Pseudomonas paraeruginosa</i>. NCBI reference sequence: NZ_CP020560.1.
+
Polyphosphate kinase 2(PPK2) from <i>Pseudomonas paraeruginosa</i>. NCBI reference sequence: NZ_CP020560.1:c164262-163366
  
 
===References===
 
===References===

Revision as of 11:06, 27 September 2024


PolyP <->Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.


Source

Polyphosphate kinase 2(PPK2) from Pseudomonas paraeruginosa. NCBI reference sequence: NZ_CP020560.1:c164262-163366

References

Zhang, H., Ishige, K., & Kornberg, A. (2002). A polyphosphate kinase (PPK2) widely conserved in bacteria. Proceedings of the National Academy of Sciences, 99(26), 16678-16683. doi:10.1073/pnas.262655199

Neville, N., Roberge, N., & Jia, Z. (2022). Polyphosphate Kinase 2 (PPK2) Enzymes: Structure, Function, and Roles in Bacterial Physiology and Virulence. International Journal of Molecular Sciences, 23(2), 670. doi:10.3390/ijms23020670