Difference between revisions of "Part:BBa K5034205:Design"
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===References===
 
===References===
  
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ZHANG H, ISHIGE K, KORNBERG A 2002. A polyphosphate kinase (PPK2) widely conserved in bacteria. Proceedings of the National Academy of Sciences [J], 99: 16678-16683.
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Zhang, H., Ishige, K., & Kornberg, A. (2002). A polyphosphate kinase (PPK2) widely conserved in bacteria. Proceedings of the National Academy of Sciences, 99(26), 16678-16683. doi:10.1073/pnas.262655199

Revision as of 10:46, 27 September 2024


PolyP <->Pi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.


Source

Polyphosphate kinase 2(PPK2) from Pseudomonas paraeruginosa. NCBI reference sequence: NZ_CP020560.1.

References

Zhang, H., Ishige, K., & Kornberg, A. (2002). A polyphosphate kinase (PPK2) widely conserved in bacteria. Proceedings of the National Academy of Sciences, 99(26), 16678-16683. doi:10.1073/pnas.262655199