Difference between revisions of "Part:BBa K5299121"
Mveligratli (Talk | contribs) |
Mveligratli (Talk | contribs) |
||
| Line 6: | Line 6: | ||
Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-β-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1]. | Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-β-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1]. | ||
| + | <html> | ||
| + | <center> | ||
| + | <figure> | ||
| + | <img class="image" src='https://static.igem.wiki/teams/5299/myrto/thiamine-1.png' width="700" height="600"> | ||
| + | <figcaption><center><b>Figure 1: Thiamine biosynthetic pathway.</b></center></figcaption> | ||
| + | </figure></center></html> | ||
<h1>Use</h1> | <h1>Use</h1> | ||
This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].<br> | This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].<br> | ||
Latest revision as of 08:58, 27 September 2024
THI20 protein (V. dahliae)
Biology
THI20 (phosphomethylpyrimidine kinase) (XM_009655087.1) has a role in V. dahliae's thiamine biosynthesis pathway.
Thiamine consists of a pyrimidine (2-methyl-4-amino-5-hydroxymethylpyrimidine, HMP) and a thiazole (4-methyl-5-β-hydroxyethyl thiazole, HET). VdTHI20 plays a role in the pyrimidine synthesis branch by phosphorylating HMP to HMP-P and HMP-P to HMP-PP. It affects colonisation, conidia germination and production, and hyphae morphology [1].
Use
This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [1].
We designed this part to test if our bacteria could produce dsRNA targeting this gene.
References
[1] Qin, T., Hao, W., Sun, R., Li, Y., Wang, Y., Wei, C., … Wang, Q. (2020). Verticillium dahliae VdTHI20, involved in pyrimidine biosynthesis, is required for DNA repair functions and pathogenicity. International Journal of Molecular Sciences, 21(4), 1378. doi:10.3390/ijms21041378 Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 64
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]