Difference between revisions of "Part:BBa K5241001"
Line 5: Line 5:
 
Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.
 
Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.
  
−
Gene Circuit Diagram:
+
<span style="font-size: 150%; font-weight: bold;">Gene Circuit Diagram:</span>
  
 
<html>
 
<html>
Line 15: Line 15:
 
Source: Pseudomonas aeruginosa
 
Source: Pseudomonas aeruginosa
  
−
Result：Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm
+
<span style="font-size: 150%; font-weight: bold;">Result：</span>
 +
 
 +
Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm
  
 
<html>
 
<html>

Revision as of 08:32, 27 September 2024


Pahdd

Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.

Gene Circuit Diagram:

Source: Pseudomonas aeruginosa

Result：

Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal BglII site found at 936
    Illegal XhoI site found at 869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 1000
    COMPATIBLE WITH RFC[1000]