Difference between revisions of "Part:BBa K5034219"

 
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===Usage and Biology===
 
===Usage and Biology===
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The PAP gene from Acinetobacter johnsonii is cloned into the PYYDT plasmid. This composite part is designed to facilitate the reversible conversion of inorganic polyphosphate (PolyP) and adenosine monophosphate (AMP) to adenosine diphosphate (ADP). The PAP enzyme plays a crucial role in phosphate and energy metabolism.
  
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<span class='h3bb'>Sequence and Features</span>
 
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===Sequence and Features===
 
<partinfo>BBa_K5034219 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5034219 SequenceAndFeatures</partinfo>
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===Construct features===
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Promoter: Constitutive promoter for continuous expression. We use tac promoter in our experiment.
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PAP Coding Sequence: Encodes the polyphosphate:AMP phosphotransferase enzyme.
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Terminator: Efficient transcription terminator to ensure proper mRNA processing. We use T7Te terminator in our experiment.
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===Experimental Characterization and results===
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Using molybdate assays to determine Pi concentration and found that PAP do not have a good capacity to polymerise phosphorus (but better than PPN1 and PPX).
  
  

Revision as of 08:25, 27 September 2024


Poly P + AMP-> ADP

aaa

Usage and Biology

The PAP gene from Acinetobacter johnsonii is cloned into the PYYDT plasmid. This composite part is designed to facilitate the reversible conversion of inorganic polyphosphate (PolyP) and adenosine monophosphate (AMP) to adenosine diphosphate (ADP). The PAP enzyme plays a crucial role in phosphate and energy metabolism.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4981
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4981
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2834
    Illegal NotI site found at 4987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4981
    Illegal BglII site found at 3580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4981
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4981
    Illegal XbaI site found at 4996
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 4244
    Illegal NgoMIV site found at 4527
    Illegal AgeI site found at 402
  • 1000
    COMPATIBLE WITH RFC[1000]

Construct features

Promoter: Constitutive promoter for continuous expression. We use tac promoter in our experiment. PAP Coding Sequence: Encodes the polyphosphate:AMP phosphotransferase enzyme. Terminator: Efficient transcription terminator to ensure proper mRNA processing. We use T7Te terminator in our experiment.

Experimental Characterization and results

Using molybdate assays to determine Pi concentration and found that PAP do not have a good capacity to polymerise phosphorus (but better than PPN1 and PPX).