Difference between revisions of "Part:BBa K5241001"
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Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.
 
Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.
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Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm
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<!-- Add more about the biology of this part here
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5241001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5241001 SequenceAndFeatures</partinfo>
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Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm
 
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https://static.igem.wiki/teams/5241/wiki/parts-static/bba-k5241001-img01.png
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 06:03, 27 September 2024


Pahdd

Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin synthesis.

Based on Pseudomonas aeruginosa PAO1 [gene=hppD] [protein=4-hydroxyphenylpyruvate dioxygenase], a Pahdd-6xH gene with a 6xH tag is synthesized in pET-21b(+), with the anticipated protein localization within the bacterial cytoplasm

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal BglII site found at 936
    Illegal XhoI site found at 869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
    Illegal EcoRI site found at 227
    Illegal EcoRI site found at 701
    Illegal PstI site found at 932
  • 1000
    COMPATIBLE WITH RFC[1000]