Difference between revisions of "Part:BBa K5152010"

Latest revision as of 00:09, 27 September 2024

5' 20 nt overlapping region for pUC19 PstI site

We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using PstI and EcoRI restriction enzymes.

We also designed a 20 nt overlapping region at the 3' end for the EcoRI site on the pUC19 vector (BBa_K5152011).

We verified that these regions allow for successful and effective assembly.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K5152010 short</partinfo>
-We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using EcoRI and PstI restriction enzymes.
+We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using PstI and EcoRI restriction enzymes.
-We also designed a 20 nt overlapping region at the 3' end for the PstI site on the pUC9 vector.
+We also designed a 20 nt overlapping region at the 3' end for the EcoRI site on the pUC19 vector (<partinfo>K5152011</partinfo>).
 We verified that these regions allow for successful and effective assembly.
@@ Line 11: / Line 12: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'><b>Sequence and Features</b></span>
 <partinfo>BBa_K5152010 SequenceAndFeatures</partinfo>