Difference between revisions of "Part:BBa K245116:Design"
(→‎Source)
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
the part was multiplied using PCR
+
The part was multiplied by PCR from GyrB-L20-MD2/pFLAG-CMV3.
  
 
===References===
 
===References===

Revision as of 18:22, 21 October 2009

GyrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.


Source

The part was multiplied by PCR from GyrB-L20-MD2/pFLAG-CMV3.

References