Difference between revisions of "Part:BBa K245046:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K245046=== | ===Applications of BBa_K245046=== | ||
| + | CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site , cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 18:13, 21 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K245046
CcdB serves as positive selection marker since it is lethal to almost all BioBrick cell strains (E.coli DB3.1 strain is an exeption). While inserting BioBrick parts in plasmid, there exist a possibility of contamination of ligation mixture with uncut vector. If vector in which one plans to insert BioBrick part contains ccdB domain in multiple cloning site , cells that were transformed with unmodified vector express ccdB protein which results in cell death. For this reason any vector containing ccdB could only be propagated in resistant cell strains like DB3.1.
User Reviews
UNIQ6e1d50d85d25c690-partinfo-00000000-QINU UNIQ6e1d50d85d25c690-partinfo-00000001-QINU