Difference between revisions of "Part:BBa K195624"

 
 
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<partinfo>BBa_K195624 short</partinfo>
 
<partinfo>BBa_K195624 short</partinfo>
  
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This main part of the time delay device.  
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This device is produces damped oscillations between TetR and CII.
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The promoter pRE could be induced by BBa_K116602,the inducer CII, which results in the production of the TetR and green fluorescent protein. TetR could repress the regulatory promoter BBa_R0040 pTet, which results in the lower production of the inducer CII.  
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The [[Part:BBa_K116603|pRE]] can be activated by [[Part:BBa_K116602|CII]], which in turn produces [[Part:BBa_C0040|TetR]] and GFP. The TetR then represses the promoter [[Part:BBa_R0040|pTet]] resulting in slower production of CII.
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For testing this part, we use the [[Part:BBa_K195622|CII/TetR Damped Oscillator With GFP Reporter (I)]] and use GFP to measure the oscillation.  
  
 
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Latest revision as of 18:00, 21 October 2009

CII/TetR Damped Oscillator (I)

This device is produces damped oscillations between TetR and CII. The pRE can be activated by CII, which in turn produces TetR and GFP. The TetR then represses the promoter pTet resulting in slower production of CII.

For testing this part, we use the CII/TetR Damped Oscillator With GFP Reporter (I) and use GFP to measure the oscillation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]