Difference between revisions of "Part:BBa K5036015"
(→Software Characterization) |
|||
Line 26: | Line 26: | ||
lang=EN style='padding-bottom:30px;font-size:11.0pt;line-height:115%'>This figure confirmed that The first nanobody-MMP9 interaction scored a binding stability (ΔG) of -12.3 kcal mol-1 which is considered a high binding stability | lang=EN style='padding-bottom:30px;font-size:11.0pt;line-height:115%'>This figure confirmed that The first nanobody-MMP9 interaction scored a binding stability (ΔG) of -12.3 kcal mol-1 which is considered a high binding stability | ||
. </span></p></div></html> | . </span></p></div></html> | ||
+ | |||
+ | Then we compared the binding stability between different nanobodies to MMP9 | ||
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/part-software/binding-of-complexes.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='padding-bottom:30px;font-size:11.0pt;line-height:115%'>This figure shows that NB1 and NB3 have higher affinity over the NB2 which make them candidates to be used in our switch | ||
+ | . </span></p></div></html> | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 14:17, 25 September 2024
Nanobody1
Part Description
nanobodies are a unique type of antibody derived from camelids. These single-domain antibodies are significantly smaller and more stable, making them ideal for applications in diagnostics and therapeutics. Nanobodies can be used to develop highly sensitive and specific tests for a variety of diseases, including cancer and autoimmune disorders. Their compact size and stability also make them promising candidates for targeted drug delivery and other biomedical applications
Usage
we insert nanobodies to reach the conditioned circular RNA by mediating the attachment between NSP3 and the MCP of the aptamer
Software Characterization
We Started by making a decision about which Nanobodies are candidates to be used in our project. We had to nominate 2 of 3 available nanobodies for the MMP-9 so we started by validating the nanobodies binding affinity to the MMP-9
MMP9-NB1 complex
This figure confirmed that The first nanobody-MMP9 interaction scored a binding stability (ΔG) of -12.3 kcal mol-1 which is considered a high binding stability .
Then we compared the binding stability between different nanobodies to MMP9
This figure shows that NB1 and NB3 have higher affinity over the NB2 which make them candidates to be used in our switch .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]