Difference between revisions of "Part:BBa K5115019"
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__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
 +
This composite part is composed of hypF and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hypF is a subunit of the hydrogenase made up by hox and hyp, get details about hypF in  [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)]. This composite part will finally be used in [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)]for further experiment.
  
 
===Usage and Biology===
 
===Usage and Biology===
 +
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
  
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===Characterization===
 
  
  

Revision as of 14:15, 25 September 2024


ribozyme+RBS+hypF+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of hypF and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hypF is a subunit of the hydrogenase made up by hox and hyp, get details about hypF in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 499
    Illegal XhoI site found at 2338
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
    Illegal NgoMIV site found at 325
    Illegal NgoMIV site found at 349
    Illegal NgoMIV site found at 710
    Illegal NgoMIV site found at 2110
    Illegal NgoMIV site found at 2510
  • 1000
    COMPATIBLE WITH RFC[1000]


References