Difference between revisions of "Part:BBa K133071"

 
 
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<partinfo>BBa_K133071 short</partinfo>
 
<partinfo>BBa_K133071 short</partinfo>
  
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Cloned into pSB1AK3 vector.
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Urease B (UreB) is one of the main ''H. pylori'' antigens. It is a subunit of enzyme urease, composed of A and B subunit that neutralizes the pH around the microorganism by decomposing urea into ammonia and carbon dioxide, thus enabling ''H. pylori'' to survive in the acidic gastric environment. Application of this antigen should elicit production of neutralizing antibodies thus inhibiting enzymatic activity of urease, which could lead to the eventual eradication of bacteria.
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===Additional Information===
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CUHK-HongKong-SBS 2024
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UreB is a member of the metallo-dependent hydrolases superfamily and requires a nickel (Ni) cation as a cofactor to perform its enzymatic activity. Each subunit of UreB associates with two nickel ions.
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===References===
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1. UniProt. (2024). Q9AFB1 · Q9AFB1_HELPX. Uniprot.org. https://www.uniprot.org/uniprotkb/Q9AFB1/entry
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:05, 25 September 2024

UreB

Urease B (UreB) is one of the main H. pylori antigens. It is a subunit of enzyme urease, composed of A and B subunit that neutralizes the pH around the microorganism by decomposing urea into ammonia and carbon dioxide, thus enabling H. pylori to survive in the acidic gastric environment. Application of this antigen should elicit production of neutralizing antibodies thus inhibiting enzymatic activity of urease, which could lead to the eventual eradication of bacteria.


Additional Information

CUHK-HongKong-SBS 2024

UreB is a member of the metallo-dependent hydrolases superfamily and requires a nickel (Ni) cation as a cofactor to perform its enzymatic activity. Each subunit of UreB associates with two nickel ions.

References

1. UniProt. (2024). Q9AFB1 · Q9AFB1_HELPX. Uniprot.org. https://www.uniprot.org/uniprotkb/Q9AFB1/entry

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1013
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 235
    Illegal AgeI site found at 1413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 121