Difference between revisions of "Part:BBa K195622"

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<partinfo>BBa_K195622 short</partinfo>
 
<partinfo>BBa_K195622 short</partinfo>
  
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This part is a time delay device using the regulatory promoter and the interaction between the inducer, repressor and regulatory promoter. The promoter pRE could be induced by BBa_K116602,the inducer CII, which results in the production of the TetR and green fluorescent protein. TetR could repress the regulatory promoter BBa_R0040 pTet, which results in the lower production of the inducer CII.  
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This device is produces damped oscillations between TetR and CII.
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The [[Part:BBa_K116603|pRE]] can be activated by [[Part:BBa_K116602|CII]], which in turn produces [[Part:BBa_C0040|TetR]] and GFP. The TetR then represses the promoter [[Part:BBa_R0040|pTet]] resulting in slower production of CII.
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This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.
  
 
== Modeling Data ==
 
== Modeling Data ==
  
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[[Image:Er3tAr2tEr7t.png|640px]]
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[[Image:Er3tAr2tEr7t.png|640px]]
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*'''Figure 1.''' This Graph is the modeling of our CII/TetR damped oscillator. There are more details on 2009 NYMU-Taipei iGEM team Wiki page.
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** [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal/Modeling Modeling data]
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*'''Figure 1.''' This Graph is the modeling of our pTet/CII damped oscillator. There is more detail on 2009 NYMU-Taipei iGEM team Wiki page.
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** [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal/Modeling]
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** [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal#Graph_of_Experiments Experiment data]  
 
** [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal#Graph_of_Experiments Experiment data]  
  

Latest revision as of 17:27, 21 October 2009

CII/TetR Damped Oscillator With GFP Reporter (I)

This device is produces damped oscillations between TetR and CII. The pRE can be activated by CII, which in turn produces TetR and GFP. The TetR then represses the promoter pTet resulting in slower production of CII.

This design was based on the design of a relaxation oscillator (two component oscillator). This design does not produce sustained oscillations like a normal oscillator (relaxation based or otherwise) because of the two oscillating effectors not being able to directly affect each other.

Modeling Data

Er3tAr2tEr7t.png

  • Figure 1. This Graph is the modeling of our CII/TetR damped oscillator. There are more details on 2009 NYMU-Taipei iGEM team Wiki page.
    • [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal/Modeling Modeling data]
    • [http://2009.igem.org/Team:NYMU-Taipei/Project/Removal#Graph_of_Experiments Experiment data]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2141