Difference between revisions of "Part:BBa K191004"
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===Usage and Biology===
 
===Usage and Biology===
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This part permits characterization of part <partinfo>BBa_K191003</partinfo> by fluorescence intensity. For complete genetic circuit, see [[Part:BBa_K191003:mainpage]]
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This part permits characterization of part <partinfo>BBa_K191003</partinfo> as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see [https://parts.igem.org/Part:BBa_K191003 here].
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Latest revision as of 17:13, 21 October 2009

TRP promoter - RBS - RFP - Term

Part BBa_K191004 consists of the Trp promoter followed by the RFP gene. In E. coli, the Trp promoter is situated in front of the Trp operon, which contains the genes necessary for the tryptophan biosynthetic pathway. In brief, genes placed after the Trp promoter should be repressed in the presence of tryptophan.

Usage and Biology

This part permits characterization of part BBa_K191003 as it contains Trp promoter sequence, to which the fused protein LovTAP can bind. For complete genetic circuit, see here.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 43
    Illegal SpeI site found at 51
    Illegal AgeI site found at 666
    Illegal AgeI site found at 778
  • 1000
    COMPATIBLE WITH RFC[1000]