Difference between revisions of "Part:BBa K265002:Design"

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===Design Notes===
 
===Design Notes===
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Signal sequence put in Tom Knight's format for fusion proteins.
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Since we expect that this part will be used in the context of a fusion protein, we have modified this protein to be consistent with BBF RFC-12 Standard. We have submitted this part to the part registry as part BBa_K265002.  Sequence taken from first 37 amino acids in phaZ1 gene.  The signal peptide cleavage site is predicted between Ala37 and Leu38 therefore target protein will get cleaved off if fused after Ala37.  Target proteins were seen to make a halo of translucence around cells on an opaque background denoting secretion of target protein.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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(1)  Se Whan Park, Moon Gyu Chung, Hwa Young Lee, Jeong Yoon Kim, and Young Ha Rhee. (2008). Stable expression and secretion of the polyhydroxybutyrate depolymerase of Paucimonas lemoignei in Escherichia coli.  J. Microbiology. 46(6). pp. 662-669.

Latest revision as of 17:12, 21 October 2009

Proteolytic Cleavage Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since we expect that this part will be used in the context of a fusion protein, we have modified this protein to be consistent with BBF RFC-12 Standard. We have submitted this part to the part registry as part BBa_K265002. Sequence taken from first 37 amino acids in phaZ1 gene. The signal peptide cleavage site is predicted between Ala37 and Leu38 therefore target protein will get cleaved off if fused after Ala37. Target proteins were seen to make a halo of translucence around cells on an opaque background denoting secretion of target protein.

Source

Synthesized from Mr. Gene.

References

(1) Se Whan Park, Moon Gyu Chung, Hwa Young Lee, Jeong Yoon Kim, and Young Ha Rhee. (2008). Stable expression and secretion of the polyhydroxybutyrate depolymerase of Paucimonas lemoignei in Escherichia coli. J. Microbiology. 46(6). pp. 662-669.