Difference between revisions of "Part:BBa K5115015"
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The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
 
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
  
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===Characterization===
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Revision as of 03:30, 25 September 2024


ribozyme+RBS+hoxW+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of hoxW and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hoxW is a subunit of the hydrogenase made up by hox and hyp, get details about hoxW in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 259
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References