Difference between revisions of "Part:BBa K5115012"
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__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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This composite part is composed of hoxU and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hoxU is a subunit of the hydrogenase made up by hox and hyp, get details about hoxU in  [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)]. This composite part will finally be used in [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)]and [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067(mineral, F module)]for further experiment.
  
 
===Usage and Biology===
 
===Usage and Biology===
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+
The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM].
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===Characterization===
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Revision as of 02:36, 25 September 2024


ribozyme+RBS+hoxU+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of hoxU and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hoxU is a subunit of the hydrogenase made up by hox and hyp, get details about hoxU in BBa_K5115020(hox and hyp operon). This composite part will finally be used in BBa_K5115066(mineral, U module)and BBa_K5115067(mineral, F module)for further experiment.

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 485
    Illegal BglII site found at 563
    Illegal XhoI site found at 493
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 446
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 267
    Illegal BsaI site found at 429
    Illegal SapI.rc site found at 378


References